GENOTIPE DAN SUBTIPE VIRUS HEPATITIS B PENDERITA YANG TERINFEKSI KRONIK AKTIF

Abstract

Chronic activivity of Hepatitis B Virus (HBV) infection can lead to liver cirrhosis or hepatocellular carcinoma. The objective of this
study was to know by analyzing the distribution of HBV genotypes and subtypes from hepatitis B patients suffering from chronic active
hepatitis B infection in Surabaya. The HBV genotypes were determined by comparing the S gene sequences to those kept in the Gene
Bank. The phylogenetic tree was constructed by means of the unweighted-pair group method using arithmetic averages. Furthermore,
the subtypes were deduced based on the prediction of amino acid residues 116 to 183 of HBsAg on multiple sequences alignment with
ClustalW2. This study involved 20 sera obtained from patients suffering chronic active hepatitis B infection. After PCR and sequencing,
it was found that 13 samples could be used for sequence analysis. The results showed that all sequences were clustered into HBV genotype
B. The subtype adw2 was identified from 12 of 13 sequences, whereas one (1) belonged to ayw1. The subtype adw2 is most prevalent in
Indonesia, namely in the islands of Sumatra, Java, South Kalimantan, Bali, Lombok, Ternate, and Morotai, while ayw1 is found in the
islands of Nusa Tenggara and Moluccas. Based on this study, it was found that the patients with HBV subtype adw2 were from Surabaya
, whereas with ayw1 was from Nusa Tenggara. It can be concluded that the HBV infected patients with chronic active hepatitis B in
Surabaya have the genotype B with subtype adw2 which was originally from Surabaya, whereas, ayw1 was a patient originally from
Nusa Tenggara.