MALDI-TOF DAN SELDI-TOF MASS SPECTROMETRY DENGAN THROUGHPUT TINGGI UNTUK ANALISIS PROTEOMIK PROFIL PROTEIN DARI PETANDA BIOLOGIS
DOI:
https://doi.org/10.24293/ijcpml.v22i2.1126Keywords:
MALDI-TOF, SELDI-TOF, mass spectrometry, high throughput, proteomic, protein profiling, biomarkerAbstract
In recent years, proteomic approach has been widely used for diagnosing the diseases and matrix assisted laser desorption /
ionization- time of the flight (MALDI-TOF) mass spectrometry and its modification, surface enhanced laser desorption /ionization-time
of flight (SELDI-TOF) mass spectrometry have became very promising diagnostic tools. High throughputs and relative simplicity of these
technologies attracted the researchers to know the biomarkers of specific diseases by analyzing specimens of serum/plasma and other
body fluids. Analyzing plasma specimens by MALDI/SELDI TOF mass spectrometry provides new information especially about small
protein and peptides in high abundance. Protein profilings, resulting by these technologies provide higher sensitivity and specificity values
than current biomarkers. Knowing how these principle tools work and its promising application to early detection of specific diseases is
the aim of writing this paper.