GLUT 4 DI JARINGAN ADIPOSA (GLUT 4 in Adipose Tissue)

Abstract

The decreasing of glucose transporter 4 (GLUT 4) in adipose tissue of diabetic and obesity patients are associated with
hyperinsulinaemia and insulin resistance. The adipose tissue can be used as therapeutic targets in the treatment of Diabetes Mellitus
(DM). Visceral adipose tissue has different morphology and functional with subcutaneous adipose tissue and Vitamin D has been known
to have contributed in DM. The aim of this study is to know the role of cholecalciferol on the expression of GLUT 4 in subcutaneous and
visceral adiposity of diabetic rats by elucidated in those tissues. The subjects of the study consisted of nineteen male diabetic rats of Wistar
strain, which were divided into control group (K) and three (3) treatment groups (X1, X2 and X3). In order to induce the condition of
DM, the animals were fed with high fat diet for three (3) weeks and administered a single intraperitoneal injection of streptozotocin (35
mg/kgBW) at the end of the second week. Cholecalciferol were administered with doses of 6.25 μg/kgBW in X1, 12.5 μg/kgBW in X2
and 25 μg/kgBW in X3 per oral everyday within 14 days. The subcutaneous and visceral adipose tissues of the subjects were processed
into histological slides with immunohistochemistry staining. The data were analyzed by one way ANOVA test and paired t-test (α= 0.05,
significance p<0.05). In this study was found the significance of GLUT 4 expression in subcutaneous adiposity (p=0.035) is different
between the groups, the differences were found between group K and groups X1, X2 and X3. Also there were found the significance different
of GLUT 4 expression in subcutaneous adiposities compared with visceral adiposities in all treatment groups (p>0.05). Based on this
study it can be concluded that cholecalciferol could increase the expression of GLUT 4 in the subcutaneous adiposity, but not in visceral
adiposity of the diabetic rats.