PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes)

Authors

  • Maimun Z Arthamin
  • Nunuk S Muktiati
  • Triwahju Astuti
  • Tri Yudani M Raras
  • Didit T Setyo Budi
  • Francisca S. Tanoerahardjo

DOI:

https://doi.org/10.24293/ijcpml.v21i3.1275

Keywords:

38-kDa recombinant protein, Mycobacterium tuberculosis, IL-2, IL-4 CD3 T lymphocytes

Abstract

Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up to
now, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection so
the development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. The
purpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellular
immune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research on
cultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. All
PBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and without
antigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TB
contacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. The
highest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].
Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthy
subjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.

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Submitted

2018-04-17

Accepted

2018-04-17

Published

2018-04-18

How to Cite

[1]
Arthamin, M.Z., Muktiati, N.S., Astuti, T., Raras, T.Y.M., Budi, D.T.S. and Tanoerahardjo, F.S. 2018. PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes). INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 21, 3 (Apr. 2018), 244–249. DOI:https://doi.org/10.24293/ijcpml.v21i3.1275.

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