Differences of Bone Marrow Features and BCR-ABL Variants in Chronic Granulocytic Leukemia Post Tyrosine Kinase Inhibitor Therapy

Wivina Riza Devi, M Darwin Prenggono, Purwanto AP, Imam B


Chronic Granulocytic Leukemia (CGL) occurs due to chromosomal translocation (9;22) known as Philadelphia
chromosome. p210 BCR-ABL1 oncogenes are classified into b2a2 and b3a2 transcripts which possibly lead to different
clinical manifestations and response to therapy. This study was aimed to prove that there is a difference in bone marrow
features and BCR-ABL between remissive and resistant CGL after Tyrosine Kinase Inhibitor (TKI) therapy. This research was
an observational study with a cross-sectional design carried out at Ulin Hospital Banjarmasin on 32 subjects. BCR ABL was
detected by using PCR and bone marrow features were assessed by using bone marrow aspiration technique. The difference
between bone marrow features and BCR-ABL variants was analyzed by using the T-test (p < 0.005) and Chi-Square
(p < 0.005), respectively. There was a difference of BCR-ABL variants with p=0.091 and characterized by M:E ratio (p=0.124),
myeloblast count (p=0.063), and eosinophil count (p=0.055). Also, there was a difference of bone marrow cellularity
(p=0.000) and basophil count (p=0.016) between remissive CGL and resistant CGL patients. There was no difference in BCR
ABL variants, myeloblast count and eosinophil count between remissive CGL and resistant CGL patients. However, there was
different of bone marrow cellularity and basophil count between remissive CGL and resistant CGL patients.


Chronic granulocytic leukemia, BCR-ABL variants, bone marrow

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DOI: http://dx.doi.org/10.24293/ijcpml.v26i2.1457


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