CORRELATION OF SERUM CYTOKINE INTERLEUKIN-6, TNF- α PROCALCITONIN AND LEUKOCYTE COUNT IN PATIENTS WITH SUSPECTED SEPSIS

Erfina Lim, Jusak Nugraha

Abstract


Introduction. Sepsis is a cause of non-cardiac death in the hospital. Early and rapid diagnosis of sepsis patients is a challenge to increase the expectancy of life. IL-6 and TNF-α are groups of proinflammatory cytokines that initiate an initial inflammatory response. Procalcitonin is a specific marker of bacterial infection. This study aimed to analyze the correlation of serum cytokine IL-6, TNF-α, procalcitonin and leukocyte count in  suspected sepsis patients.

Methods. This was a cross-sectional observational study.  The study subjects consisted of 45 patients with suspected sepsis who were examined for procalcitonin level > 0.5 ng/mL. Procalcitonin examination by  ELFA (VIDAS) , IL-6 and TNF- α used U-CyTech Human Elisa kit (Bioscience, INC) and leukocyte counts with SYSMEX-XN 1000.

Results. The levels of IL-6 ranged 0 pg/mL – 73.29 ng/mL (mean 29.43 ng/mL). The values for TNF- α were 0 pg/mL – 390.5 pg/mL (mean 27.62 pg/mL). The mean value of  leukocytes was 20,139/ μL. There was no correlation between leukocyte counts with IL-6 ( p = 0.798 and r = 0.040),  TNF- α (p = 0.304 and r = -0.160),  Procalcitonin ( p = 0.323 and r = 0.154). There was no correlation between  IL-6 levels with  TNF-α levels (p = 0.871 and r = -0.025), procalcitonin levels ( p = 0.466 and r = 0.112).  There was a weak negative correlation between TNF- α  level and procalcitonin levels ( p=0.006 and r = -0.403)

Conclusion. There was a weak negative correlation between the level of procalcitonin with TNF- α in suspected sepsis patients.


Keywords


Sepsis, Procalcitonin, IL-6, TNF- α

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DOI: http://dx.doi.org/10.24293/ijcpml.v26i2.1463

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