IgA ANTI-DENGUE PROFILE IN SAMPLES WITH POSITIVE DENGUE PCR OR NS1
DOI:
https://doi.org/10.24293/ijcpml.v25i1.1483Keywords:
Dengue virus infection, IgA anti-dengue, dengue virus serotype, type of infection dengue virus, dengue virus severity, AIM Dengue IgA Assure Rapid TestAbstract
Dengue Virus Infection (DVI) causes several clinical manifestations and requires varied diagnostic instruments. IgA anti-dengue as one of the diagnostic markers of DVI is suspected to have a shorter lifespan and greater sensitivity in detecting secondary infections compared to IgM anti-dengue. This study was conducted using 34 sera with positive RT-PCR or NS1 dengue virus. Samples were examined by a reverse flowimmunochromatographic method using AIM Dengue IgA Assure Rapid Test and will be analyzed its profile toward the day of fever, serotype, severity, platelet count, and type of infection. The overall sensitivity of IgA anti-dengue was 61.76% (n=34); in which IgA anti-dengue detected 14.29% primary and 66.67% secondary cases. IgA anti-dengue detected DEN1, DEN2, DEN3, and Mixed DEN1 – DEN3 virus serotype respectively 55.56%, 22.22%, 16.67%, and 5.56% (n=20). The day of fever was dominated by day-4 and day-5 respectively 28.57% (n=21). IgA anti-dengue was detected in DD, DHF grade I, II, and III 42,86%, 28.57%, 19.05%, and 9.52% (n=21) respectively. IgA anti-dengue detected in all levels of platelet count, it detected 60% in < 50,000 cell/mm3, 30% in 50,000 - 100.000 cell/mm3 and 10% in > 100,000 cell/mm3 platelet count sample (n=20). In conclusion, IgA anti-dengue showed a good performance, applicable as a diagnostic marker of DVI.Downloads
References
World Health Organization. Dengue guidelines for diagnosis, treatment, prevention, and control. 2011 [cited on December 12, 2017]. Available at: http:// www.who.int.
Aaron L, Jeremias P, and Matthias T. Outside of normal limits: False positive/negative anti TG2 autoantibodies. International Journal of Celiac Disease. 2015; 3(3): xx. [cited on June 29, 2016] Available at http://pubs.sciepub.com/ijcd/3/3/4.
Primadi O, Sitohang V, Budijanto D, and Soenardi TA. Data dan informasi tahun 2014 (profil kesehatan Indonesia). Kementerian Kesehatan Republik Indonesia. 2015 [cited on December 13, 2017]. Available at: http:// www.pusdatin.kemkes.go.id.
Gubler DJ. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev. 1998; 11: 480–96. [cited on December 13, 2017]. Available at: https://www.ncbi.nlm.nih.gov/pubmed/9665979.
Chen WJ, Hwang KP, Fang AH. Detection of IgM antibodies from cerebrospinal fluid and sera of dengue fever patients. Southeast Asian J Trop Med Public Health. 1991; 22:659–63. [cited on December 13, 2017]. Available at: https://www.ncbi.nlm.nih.gov/pubmed/1820657.
Ahmed F, Mursalin H, Alam MT, Amin R, Sekaran SD, et al. Evaluation of assure dengue IgA rapid test using dengue positive and dengue negative samples. Diagnostic Microbiology and Infectious Disease. 2010; 68: 339-44. [cited on December 13, 2017].
Tan YY, Sekaran SD, Seok MW, Ahmed F, Hossain A, and Sil BK. Development of Assure® Dengue IgA rapid test for the detection of Anti-dengue IgA from dengue- infected patients. 2011; 3(3): 233–240. [cited on December 13, 2017]. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162809.
Resna Hermawati, Aryati, Puspa Wardhani, Triyono Erwin. Nilai diagnostik anti-dengue IgA, dan NS1 serta IgM/IgG di infeksi virus dengue. Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP&ML) 2014; 21(1): 82–89. [cited on December 13, 2017].
Aryati, Wardhani P. Profil virus dengue di Surabaya tahun 2008–2009. Indonesian Journal of Clinical Pathology and Medical Laboratory. 2010; 17(1): 21-24.
De Decker S, Vray M, Sistek V, Labeau B, Enfissi A, Rousset D, Matheus S. Evaluation of the diagnostic accuracy of a new dengue IgA capture assay (Platelia Dengue IgA Capture, Bio-Rad) for dengue infection detection. PLoS Negl Trop Dis. 2015; 24; 9(3): 35-96. [cited on December 13, 2017]. Available at : https://www.ncbi.nlm.nih.gov/pubmed/25803718.
Ipa M, Astuti EP. Secondary infection and Den-3 serotype most common among dengue patients: A preliminary study. Health Science Indonesia, 2010; 1(1): 14–9.
Andriyoko B, Parwati I, Tjandrawati A, Lismayanti L. Penentuan serotipe virus dengue dan gambaran manifestasi klinis serta hematologi rutin pada infeksi virus dengue. Majalah Kedokteran Bandung. 2012; 44(4): 253-260.
Aryati. Demam berdarah dengue (tinjauan laboratoris). Jilid 1. Surabaya, Airlangga University Press, 2011; 101-7. [cited on December 13, 2017].
Aryati, Trimarsanto H, Yohan B, Wardhani P, Fahri S and Sasmono RT. Distribusi serotype dengue di Surabaya tahun 2012. Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP&ML), 2012; 19(1): 41-4. [cited on December 13, 2017].
Aryati, Trimarsanto H, Yohan B, Wardhani P, Fahri S, and Sasmono RT. Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia. 2013; 13: 611. [cited on December 13, 2017].
