Immunophenotyping Pattern in Childhood Acute Leukemia in the Adam Malik Hospital Medan

Putri Chadijah Tampubolon, Bidasari Lubis, Adi Koesoema Aman, Malayana R Nasution

Abstract


 

Leukemia is the most common malignancy at the age of under 15 years, with a ratio of 1 to 3 cancer cases in children.
Immunophenotyping will improve accuracy and easily provide data reproducibility. To determine the immunophenotyping
pattern in patients with acute leukemia in the Pediatric Center at the Adam Malik General Hospital, Medan. This research was
a cross-sectional study in children suffering from acute leukemia in the Pediatric Unit Adam Malik General Hospital, Medan
based on CBC, peripheral smear, bone marrow morphology, and flow cytometry immunophenotyping. Samples were
evaluated for blast morphologic and immunophenotyping was carried out. Results of morphologic observation and
immunophenotyping were compared. From 20 samples using the monoclonal antibody with flow cytometry
immunophenotyping, concordance with morphology is good (κ = 0.886). After classification, the percentage of acute
leukemia was 45% B-ALL, 35% AML, and 20% T-ALL. One of 10 samples morphologically classified as ALL but reported as
AML. Immunophenotyping has been shown to increase diagnostic accuracy and assist in establishing lines in blast cells,
which was initially merely based on morphological features.


Keywords


Aberrant, acute leukemia, flow cytometry, immunophenotyping

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DOI: http://dx.doi.org/10.24293/ijcpml.v27i1.1602

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