The Effect of Extract Bitter Melon and Snakehead Fish on Advanced Glycation End Products

Authors

  • Sari Amalia Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta
  • MI. Diah Pramudianti Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta
  • Yuwono Hadisuparto Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

DOI:

https://doi.org/10.24293/ijcpml.v27i3.1696

Keywords:

Momordica charantia, Channa striata, advanced glycation end products, type-2 diabetes mellitus

Abstract

Diabetes mellitus type-2 is a transformation of insulin function in peripheral tissues or disorders of pancreatic β cells. The combination of glycation and glucose oxidation in type 2 DM patients results in the formation of AGEs which can result in oxidative stress. One of the efforts to reduce oxidative stress is the use of natural ingredients as a source of antioxidants. For example, bitter melon (Momordica Charantia) and snakehead fish (Channa Striata) extracts. The combination of bitter melon and snakehead fish extracts against AGEs levels is still limited and has only been tested on Wistar rats and mice. This study aims to determine how much effect the combination of bitter melon and snakehead fish extracts has on AGEs levels in type-2 DM patients. This study was clinical experimental randomized double blind control trial. The experiment conducted at Purwosari Public Health Center, Surakarta and the examination of AGEs levels was in the Clinical Pathology Laboratory of Dr Moewardi Surakarta from July to August 2020. Comparative analysis if the results were normally distributed, in pairs, numerical data and consists of 2 groups then use the paired sample t-test. The results were significant if p<0,05 with a confidence interval (CI) of 95%. A total of 40 subjects consisted of 27 women and 13 men. The results of the analysis test showed significant differences in the level of AGEs in the pre- 70.78 (53.47-321.93) pg/mL and the post-39.91 (34.70-71.85) pg/mL (p=0.001) in the group that received the combination of bitter melon and snakehead fish extracts. There was an effect in AGEs levels after receiving the combination of bitter melon and snakehead fish extracts. Can be used as a supplement to reduce complications of type 2 diabetes. Further research is needed to specifically measure the AGEs products that have decreased.

Downloads

Download data is not yet available.

Author Biographies

Sari Amalia, Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

MI. Diah Pramudianti, Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

Yuwono Hadisuparto, Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

Department of Clinical Pathology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta

References

Decroli E. Diabetes melitus tipe 2. Padang, Pusat Penerbitan Bagian Ilmu Penyakit Dalam Fakultas

Kedokteran Universitas Andalas, 2019; 2.

PERKENI. Konsesus pengelolaan dan pencegahan diabetes melitus tipe 2 di Indonesia 2019. Jakarta, PB PERKENI, 2019; 3.

Subandrate. Hubungan kadar glukosa darah dengan peroksidasi lipid pada pasien diabetes melitus tipe 2. Palembang, CDK-242, 2016; 43(7): 2-3.

Jud P, Sourij H. Therapeutic options to reduce advanced glycation end products in patients with

diabetes mellitus: A review. Diabetes Research and Clinical Practice Elsevier B.V, 2018; 148: 54-63.

Phimarn W, Sunthong B, Saramunee K, Caichompoo W. Efficacy of Momordica charantia L. on blood

glucose, blood lipid, and body weight: A meta-analysis of randomized controlled trials. Pharmacognosy

Magazine, 2018; 14(56): 2-3.

Chanda R, Samadder A, Banerjee J. Anti-diabetic activity of Momordica charantia or bitter melon: A

Review. Acta Scientific Pharmaceutical Sciences, 2019; 3(5): 2581-5423.

Ningrum D, Abdulgani N. Pengaruh pemberian ekstrak ikan gabus (Channa striata) pada struktur

histologi hati mencit (Mus musculus) hiperglikemik. Jurnal Sains dan Seni Pomits, 2014; 2(1): 2337-3520.

Aljohi A, Matou-Nasri S, Ahmed N. Antiglycation and antioxidant properties of Momordica charantia. PLos One, 2016; 11(8): 1-14.

Kumari S, Dash I, Behera K. Therapeutic effect of Momordica charantia on blood glucose, lipid profile

and oxidative stress in type 2 diabetes mellitus patients: A randomised controlled trial. Journal of

Clinical and Diagnostic Research, 2018; 12(9): 1.

Soniya F, Fauziah M. Efektivitas ekstrak ikan gabus sebagai antihiperglikemik. Jurnal Penelitian Perawat Profesional, 2020; 2(1): 2.

Sunarno. Potential of glutathione antioxidant in the hippocampus repair: preliminary study on bioactive materials antiaging of snakehead fish (Channa striata) in animal models of aging. Internat. J. Sci. Eng, 2015; 8(1): 25.

Anonim. Human advanced glycation end products (AGEs) ELISA kit. Instruction version r09.3.1. 2020; Cat No. MHBS267540.

Xu W, Morford J, Mauvais-jarvis F. Emerging role of testosteron in pancreatic ?-cell function and insulin secretion. Journal of Endocrinology, 2019; 240(3): R97-R105.

Zhu H, Wang N, Han B, Li Q, Chen Y, Zhu C, Chen Y, et al. Low Sex hormone-binding globulin levels

associate with prediabetes in chinese men independent of total testosterone. PLos One, 2016; 11(9): 6-10.

Kautzky-willer A, Harreiter J, Pacini D. Sex and gender differences in risk, pathophysiology and complications of type 2 diabetes mellitus. Endocrine Reviews, 2016; 37(3): 278-316.

Asiimwe D, Mauti G, Kiconco R. Prevalence and risk factors assocciated with type 2 diabetes elderly

patients aged 45-80 years at Kanungu district. Journal of Diabetes Research, 2020; 2020: 1-5.

Riset Kesehatan Dasar (RISKESDAS). Hasil utama RISKESDAS 2018. Jakarta, Badan Penelitian dan

Pengembangan Kesehatan, 2018; 46-48.

Ambardekar N. Diabetes and alcohol. 2019. Available at: https://www.webmd.com/diabetes/guide/

drinking-alcohol (accessed 27 September, 2020).

Falck S. Diabetes, alcohol, and social drinking. 2019. Available at: ttps://www.healthline.com/health/

type-2-diabetes/facts-diabetes-alcohol (accessed 27 September, 2020).

American Addiction Centers. Alcohol use among diabetics: increased risk & treatment complications.

Available at: https://www.alcohol.org/comorbid/diabetes-and-alcoholism (accessed 27 September, 2020).

Center for Disease Control and Prevention (CDC). Estimates of diabetes and its burden in the United

States. National Diabetes Statistics Report, 2020; 1-30.

Soylar P, Kadioglu B. Kilic K. Investigation of the barriers about insulin therapy in patients with type 2

diabetes. Nigerian Journal of Clinical Practice, 2020; 23(1): 98-102.

Raghavendran S, Inbaraj L, Norman G. Reason for refusal of insulin therapy among type 2 diabetes

mellitus patients in primary care clinic in Bangalore. Journal of Family Medicine and Primary Care, 2020;

(2): 854-8.

Zhang P, Zhang H, Li X, Chen M, Wang D, Ji L. Factors and outcomes associated with discontinuation of basal insulin therapy in patients with type 2 diabetes mellitus. Endocrinol Diab Metab, 2020; 3: 1-10.

Suhendi A, Muhtadi M, Sutrisna EM. Anti-inflammatory and antidiabetic of Channa striata powder and Nephelium lappaceum fruit peel ethanolic extracts on albino wistar mice. Drug Invention Today, 2019; 12(11): 2472-75.

Muhtadi, Pangestuti. Aktivitas antidiabetes dari kombinasi ikan gabus (Channa striata) dan ekstrak

etanol buah pare (Momordica charantia L.) terhadap tikus wistar jantan yang diinduksi aloksan. University Research Colloqium, 2019; 40-47.

Downloads

Submitted

2020-09-29

Accepted

2021-05-19

Published

2021-09-21

How to Cite

[1]
Amalia, S., Pramudianti, M.D. and Hadisuparto, Y. 2021. The Effect of Extract Bitter Melon and Snakehead Fish on Advanced Glycation End Products. INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 27, 3 (Sep. 2021), 307–312. DOI:https://doi.org/10.24293/ijcpml.v27i3.1696.

Issue

Section

Articles