PENGARUH PENGAWET BEKU (CRYOPRESERVATION) TERHADAP KADAR EPIDERMAL GROWTH FACTOR (EGF) PADA SELAPUT AMNION

Abstract

to measure the difference of EgF concentration in between fresh amniotic membranes and with cryopreservation in 16 amniotic
membranes. Each amniotic membrane was divided into two parts. the first part was extracted in fresh forms and the second part
underwent cryopreservation with glycerol 50% and was stored at –80° C during 1 month before extraction. Both parts of the membrane
were extracted using ultrasonic disintegrator and concentration of EgF was measured from the obtained extract using ELISA method.
the average concentration of EgF in the fresh amniotic membrane was 122.76 ± 11.59 pg/g while the average concentration of EgF
in the amniotic membrane underwent cryopreservation was 99.34 ± 9.49 pg/g. Average degradation of EgF concentration due to
cryopreservation is 18.49% ± 10.20%. EgF concentration in fresh amniotic membrane is significantly higher than the EgF concentration
in amniotic membrane underwent cryopreservation (p = 0.000). Degradation of EgF concentration due to cryopreservation at 95%
fidence interval is 12.33% to 24.66%