UJI KESAHIHAN (VALIDITAS) PEMERIKSAAN D-DIMER CARA MENYARING KEKEBALAN (METODE IMUNOFILTRASI) DAN CARA MENGUKUR IMUNOTURBIDIMETRI

David Rustandi, Delita Prihatni, Tiene Rostini, Nina Tristina

Abstract


D-Dimer parameter is the most usefull laboratory assay to detect the present of activated coagulation. The D-Dimer fragment directly
indicate the fibrinolitic proccess whereas the increasing mark of D-Dimer is the most hemostatic parameter that was used to detect
the early stage of Disseminated Intravascular Coagulation (DIC) and has a correlation with the patient prognosis. D-Dimer assay by
immunoturbidimetric method was used to detect antigen-antibody reaction automatically and it can detect D-Dimer concentration less
than 0.5 μg/mL. The immunoturbidimetric method has a good correlation with the ELISA method, but the proccess is complicated, is
high costed, and need the competence of practical human resource. D-Dimer assay with immunofiltration method has the same principle
as immunoturbidimetric method, but it's work more simpler, low cost and does not need the competence of practical human resource.
The aim of this study is to compare the D-Dimer assay concentration between immunofltration and immunoturbidimetric method. There
were 30 plasma samples assayed with these two methods (immunoturbidimetric and immunofiltration), and then compared between
them. The samples were collected between November until December 2008 at Rumah Sakit dr. Hasan Sadikin Bandung. The validity of
D-Dimer using immunofiltration method showed sensitivity 74%, specificity 95%, predictive value of negative test (PV-) 22%, predictive
value of positive test (PV+) 95% with likelihood ratio 5.2. The conclusion of this study so far that the immunofiltration method has a
good validity for diagnosing. D-Dimer work rapidly with low cost laboratory assay than the immunoturbidimetric method.


Keywords


D-Dimer, imunoturbidimetri, imunofiltrasi, kesahihan (validitas)

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DOI: http://dx.doi.org/10.24293/ijcpml.v17i1.1049

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