Sotianingsih Sotianingsih, Soeharyo Soeharyo, Lisyani S, Guntur H


Sepsis remains a health problem because of the high related mortality and morbidity. One of the efforts is the improvement of knowledge
about manifestation and pathophysiology of sepsis at the level of molecular biology. Nitric oxide (NO) levels increased in sepsis and this is
related to the occurrence of cell apoptosis. The purpose of this study was to know and to see the clinical picture of sepsis and elevated levels of
NO in mice Balb/C which were injected with lipopolysaccharide (LPS). In this research, the mice were divided into three (3) groups, group
A were injected with saline, group B were injected with LPS 0.1 mg per mouse, while group C was injected with 0.2 mg per mouse. The
mice were then measured by clinical parameters from day 0 and day 1, and 3,5 to 7. The levels of NO were assessed by blood sampling as
much as 1 cc from the peri orbital plexus and examined by Griess method on days 1, 3, 5, 7. The results of the study showed that from the
weight parameter there was a decrease in body weight on day 1 in group B and C and the weight continued to decline until day 3. From the
temperature parameter it was shown that the temperature decreased both in group B and C on day 1 but on day 3 and so on varies in both
groups. From the piloerection parameters from day 1 to day 7 showed that there was piloerection in both groups. Related to the discharge by
peri orbital parameters and diarrhoea many variations, occurred on day 1 peri orbital discharge and diarrhoea was found, but not uniformly
in all the mice in each group. On day 5 and 7 the peri orbital discharge and diarrhoea were not visible. NO levels increased mainly on day
3 and the percentage was higher in group C and on day 5 and day 7 the levels were negative. In conclusion in this study it was shown, that
the clinical picture is striking, weight loss and increased levels of NO occurs on day 3 of the observation.


Mice, sepsis, lipopolysaccharide, clinical, nitric oxide

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