AIR GANDARUSA (Justicia gendarussa Burm. f.) DAN GAMBARAN GEN HYALURONIDASE LEWAT ANALISIS PCR

Sri Lestari Utami, Didik P. Restanto, Bambang Prajogo EW

Abstract


Gandarusa (J. gendarussa Burm. f.) is an etnomedicine which is used as a male contraceptive alternative, known to inhibit the enzyme
function of spermatozoa hyaluronidase during fertilization. One of the ideal contraceptive conditions is the safetyness (among others non
mutagenic), requiring a long research during the verification process. The early research is the gene expression of hyaluronidase mice
(M.musculus L.) testis given water fraction of the gandarusa with PCR analysis. The total RNA was isolated from the normal mice testis
(as the negative control or no treatment group) and the mice testis from the treatment groups. The treatment groups consisted of group I
and II treated with water fraction of the gandarusa 15 mg/20 gr BW and 7.5 mg/20 gr BW, subsequently, the positive control group was
also given hesperidins 1 mg/20 gr BW once a day per oral during the 1.5 times of spermatogenesis cycle (for 55 days). The results of the
study showed that (1) the cDNA fragment confirmed as the gene of hyaluronidase mice testis (with 710 bp length of nucleotide) passed
through RT-PCR at the total RNA negative control group, sequenced, isolated and alignment in the NCBI gene bank. (2) the same cDNA
fragment gene of hyaluronidase mice testis (from the negative control group) did not transcript in the treatment I and positive control
groups (where there is no band), but this gene will be transcripted in the treatment II group (where the band is emerged).


Keywords


Male contraception, gandarusa, hyaluronidase gene, PCR analysis

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DOI: http://dx.doi.org/10.24293/ijcpml.v19i2.1059

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