SARI ETANOL, ETIL ASETAT ALANG-ALANG (IMPERATA CYLINDRICA) TERHADAP SUPEROXIDE DISMUTASE (SOD)

Abstract

Oxidative stress has been considered as a mechanism involved in the pathogenesis and progression of many diseases such as:
hypercholesterolemia and atherosclerosis. The objective of this current study was to know the potential antioxidant role of ethanol and
ethyl acetate extract of reeds (Imperata cylindrica) by investigation in rats fed with a high-cholesterol diet. Imperata cylindrica is rich
in antioxidants and has a cholesterol-reducing effect. A group of twenty four male albino Wistar rats was divided into four subgroups: a
normal diet group (K0), a high-cholesterol diet group (K1), the same diet with ethanol extract of Imperata cylindrica group (K2), and the
similar diet with ethyl acetate fraction of Imperata cylindrica group (K3),. After the treatment period, the hepatic antioxidant enzyme
activity (Superoxide dismutase/SOD) was determined. The rats fed on high-cholesterol diet with supplementation exhibited a significant
elevation in SOD activity (p<0. 05), 61.6±12.4% inhibition, compared to normal animals 37.44±12.2%. The treated animals with
ethanol and ethyl acetate extract of Imperata cylindrica showed a significantly reduced the level of SOD activity (p<0.05, 29.90±5.6%
as well as a 33.49±7.4% inhibition). The level of SOD activity when restored was closer to that in normal animals, signifying reversal
of the oxidative stress. The study results showed that the treatment with Imperata cylindrica positively changed the hepatic antioxidant
enzyme activities in high fat-diet rats, and thus had potential hypocholesterolemic and antioxidant effects. Imperata cylindrica could
protect against oxidative stress linked to atherosclerosis and also decrease the atherogenic index.