PHOSPHATIDYLINOSITOL -3KINASE (PI3K) DI PERBENIHAN ADIPOSIT YANG DIPAJAN GLUKOSA TINGGI DENGAN RETINOL

Authors

  • Novi Khila Firani
  • Bambang Prijadi

DOI:

https://doi.org/10.24293/ijcpml.v22i2.1111

Keywords:

Phosphatidylinositol-3 kinase, adipocyte culture, high glucose exposure, retinol

Abstract

Retinol is one of the active forms of vitamin A. In the previous study, it was known that retinol level in serum of DM patient was
lower than in healthy people, which correlated with an increase of the glucose levels in these patients. The importance of retinol in insulin
signaling mechanisms that play a role in the pathogenesis of DM is still unknown. One of the components that play a role in insulin
signaling on adipocytes is phosphatidylinositol-3 kinase (PI3K), which encourages the translocation of glucose transporter-4 (GLUT4) to
the cell surface. The aim of this study was to know the importance of retinol therapy in the levels of PI3K enzyme on visceral adipocyte
culture with high glucose exposure (25 mM) as a model of DM in vitro by determination method. Retinol therapy was given at a dose
of 0.1 μM, 1 μM , and 10 μM. Measurement of PI3K level was done by ELISA method. The mean (SD) levels of PI3K enzyme were 1.91
(0.27), 0.94 (0.15), 1.98 (0.22), 1.69 (0.81), 2.04 (0.16) ng/mL respectively, for adipocyte cultures exposed to 5mM glucose (as a
physiological condition), 25mM glucose, and 25mM glucose concentration with doses of retinol therapy 0.1 μM, 1 μM and10 μM. The
results of this study indicated that high glucose exposure (25 mM) decreased the level of PI3K compared with adipocyte's culture on
5 mM glucose exposure. Retinol therapy with a dose of 0.1μM, 1μM and10 μM on adipocyte culture exposed with high glucose could
increase the levels of PI3K.

Downloads

Download data is not yet available.

Downloads

Submitted

2018-03-27

Accepted

2018-03-27

Published

2018-03-27

How to Cite

[1]
Firani, N.K. and Prijadi, B. 2018. PHOSPHATIDYLINOSITOL -3KINASE (PI3K) DI PERBENIHAN ADIPOSIT YANG DIPAJAN GLUKOSA TINGGI DENGAN RETINOL. INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 22, 2 (Mar. 2018), 109–113. DOI:https://doi.org/10.24293/ijcpml.v22i2.1111.

Issue

Section

Articles