FCγII (CD32) MONOSIT DI INFEKSI DENGUE PRIMER DAN SEKUNDER {FcγRII (CD32) Monocytes in Primary and Secondary Dengue Infection}

Abstract

Dengue infection is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection vary
widely, from asymptomatic until dengue shock syndrome (DSS). Antibody Dependent Enhancement (ADE) hypothesis that states that
non-neutralizing antibodies in secondary dengue infection may enhance dengue infection via Fcγ receptors is still controversial. Clinical
research shows that not all secondary infections manifest as DHF/DSS, but nearly all DHF/DSS cases are caused by secondary infection.
Allegedly, the expression of Fcγ has an effect on this incident. This study is an observational analytical study with a cross sectional design
to determine the expression of FcγRII (CD32) monocytes in patients with primary and secondary dengue infection. CD32 of monocytes
was measured using FACS Calibur with lyse no wash technique. Primary and secondary dengue infection were determined by IgM/IgG
optical density ratio using ELISA capture method. The ratio of IgM/IgG ≥1.2 was considered as primary infection, while the ratio <1.2
was considered as secondary infection. Twenty primary and 32 secondary dengue infection patients in acute phase of dengue infection
partisipated in this study. Expressions of Fcγ RII (CD32) monocytes were significantly lower in primary than secondary dengue infection
(187.825±31.584 vs 218.598±43.414 MFI; p=0.008). CD32 expressions were higher in day 3 compared to day 4 of fever.