NITRIT OKSIDA DAN VOLUME EDEMA OTAK PADA STROK PERDARAHAN DALAM OTAK DENGAN POLIMORFISME G894T (Nitric Oxide and Cerebral Edema Volume in Intracerebral Hemorrhagic Stroke with G894T Polymorphism)

Authors

  • Iskandar Zakaria
  • Arif Faisal
  • Sri Sutarni
  • Ahmad Hamim Sadewa
  • Imran Imran

DOI:

https://doi.org/10.24293/ijcpml.v22i1.1229

Keywords:

eNOS gene Polymorphism, Nitric Oxide, peri focal edema, hemorrhagic stroke

Abstract

Nitric Oxide (NO) is a vasodilator that regulates vascular smooth muscle tone. Low levels of NO can cause vasoconstriction and
hemodynamic disturbances. In stroke the levels of NO are increased. Endothelial nitric oxide synthase gene polymorphism (eNOS) is
believed to reduce levels of NO in blood. NO levels decreased in stroke patients with G894T polymorphisms of eNOS gene. Mortality rate
of hemorrhagic stroke are increased in case with increased peri focal edema volume. The mechanism of the increased of peri focal edema
volume completely unknown yet, suspected genetics factor. This study was conducted to know the correlation between the NO and peri
focal edema volume in stroke with eNOS gene G894T polymorphism by determination. The study was conducted by comparing the levels
of NO and edema volume of intra cerebral hemorrhagic stroke of 46 subjects from Neurology department of dr. Zainoel Abidin general
hospital in Banda Aceh from September 2014 through January 2015 with comparison to ischemic stroke patients the same amount.
NO levels checked with Cayman Systems kit following the protocol Griess. G894T polymorphism was determined by PCR-RFLP method.
The volume of edema was measured with semi-automatic CT volumetry. Chi Square test was used for comparison of two variables and
Spearman correlation test to assess the relationship between the NO and perifocal edema volume. The result is significant, if p value
was <0.05. The results of these study were levels of NO decreases if there were polymorphism (p=0.001). Peri focal edema volume was
increased if there were G894T polymorphism (p=0.038). The correlation between low levels of NO and increase of edema volume was
obtained p=0.040 and R=0.304. The researchers concluded that in intra cerebral hemorrhagic stroke the level of NO were decreased
and peri focal edema volume increased if there was G894T polymorphism of eNOS gene. There was a less correlation between low levels
of NO and peri focal edema volume.

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Submitted

2018-04-14

Accepted

2018-04-14

Published

2018-04-14

How to Cite

[1]
Zakaria, I., Faisal, A., Sutarni, S., Sadewa, A.H. and Imran, I. 2018. NITRIT OKSIDA DAN VOLUME EDEMA OTAK PADA STROK PERDARAHAN DALAM OTAK DENGAN POLIMORFISME G894T (Nitric Oxide and Cerebral Edema Volume in Intracerebral Hemorrhagic Stroke with G894T Polymorphism). INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 22, 1 (Apr. 2018), 87–91. DOI:https://doi.org/10.24293/ijcpml.v22i1.1229.

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