PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes)

Maimun Z Arthamin, Nunuk S Muktiati, Triwahju Astuti, Tri Yudani M Raras, Didit T Setyo Budi, Francisca S. Tanoerahardjo


Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up to
now, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection so
the development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. The
purpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellular
immune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research on
cultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. All
PBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and without
antigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TB
contacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. The
highest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].
Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthy
subjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.


38-kDa recombinant protein, Mycobacterium tuberculosis, IL-2, IL-4 CD3+T lymphocytes

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