ANALYSIS OF LACTIC AND HEMATOCRIT LEVELS OF BLOOD STORAGE IN DR. WAHIDIN SUDIROHUSODO GENERAL HOSPITAL BLOOD BANK

Authors

  • Rysna Wahyu
  • Asvin Nurulita
  • Rachmawati Muhidin

DOI:

https://doi.org/10.24293/ijcpml.v25i3.1450

Keywords:

Lactic, hematocrit, stored blood, packed red cells

Abstract

The components of Packed Red Cells (PRC) are transfused to patients in order to repair oxygen transportation to tissues. The blood is stored at 2-6oC to delay red blood cells metabolism during storage. Red blood cells undergo structural and functional changes biochemically which affect their viability and function. This is a prospective cohort study with time series design. Samples were taken from fresh blood PRC which were moved to transfer bag for approximately 20 mL, then stored in the refrigerator. Lactic acid and hematocrit levels were assessed with spectrophotometry and flow cytometry methods on day 1, day 4, and day 8 of storage in the Dr. Wahidin Sudirohusodo General Hospital Blood Bank. Statistical tests used were Friedman and Wilcoxon. Statistical results are significant if p < 0.05. Total samples were 15 fresh blood PRC. Friedman statistical test showed a significant difference in lactic level (p < 0.001) and hematocrit level (p=0.012) on day 1, day 4, and day 8 of storage. Wilcoxon test showed significantly higher lactic level between day 4 and day 1 (p < 0.01); day 8 and day 1 (p < 0.01); day 4 and day 1 of storage (p < 0.01). Hematocrit level between day 4 and day 1 (p < 0.05); day 8 and day 1 (p < 0.05) were significantly higher; day 8 and day 4 of storage (p > 0.05) showed insignificant difference. Results showed that lactic and hematocrit levels of PRC stored blood were increased according to storage duration. Packed red cells blood is recommended to be given in < 6 days for lower acidosis risk. Further studies are also recommended with a shorter interval of assessment and a bigger sample size.

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References

Sudiono. H, Iskandar. Ign, Edward. H, dkk. Transfusi Darah. Dalam: Penuntun Patologi Klinik Hematologi. Jakarta. Biro Publikasi Fakultas Kedokteran Ukrida. 2009. Hal:200-9

Bakta IM. Transfusi Darah. Dalam: Hematologi Klinik Ringkas. Jakarta. Penerbit Buku Kedokteran EGC. 2006. Hal:271-3

Prihartini D. Jenis-Jenis Komponen Darah dan Indikasi Pemberiannya. Dalam: Dasar-Dasar Transfusi Darah. Jakarta. Penerbit Fakultas Kedokteran Universitas Padjadjaran. 2011. Hal:49-54.

Rudmann SV. Blood Banking and Transfusion Medicine 2nd Edition. Churcill Livingstone Elsevier. 2005. Page: 202

Weinstein SM. Plumer's Principle & Practice of Intravenous Therapy 8th Edition. Lippincot Williams & Williams. 2006. Page: 421

Bain BJ and Lewis SM. Basic Haematological techniques. In: Practical Haematology Eleventh Edition. Churcill Livingstone Elsevier. 2012. Page: 41-2.

Soebrata RG. Penuntun Laboratorium Klinik. Jakarta. Dian Rakyat. 2002. Hal: 39-42.

Hess JR, Beyer GM. Red Blood Cell Metabolism during Storage. In : Blood banking and Transfusion Medicine. 2nd Ed., Philadelphia. Elsevier. 2007. Page:205-10.

Leitner. C Gerda, Rach. I, Horvath. M, et al. Collection and Storage of Whole Blood in Autologous Blood Predeposit in Elective Surgery Programs. International Journal og Surgery. 2006; 4(3): 179-83.

Adias. T. A. Igwe-M B and jeremiah. A Z. Storage Related Haematological and Biomedical Changes of CPDA-1 Whole Blood in A Resource Limited Setting. Journal of Blood Disorders & Transfusion.2012;3(3): 2155-9864.

Mustafa. I, Marwani Al, Nasr.MK, et al. Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM. Vol 2016. Article ID 4529434. 7 Pages. 2016

Flatt JF, Bawazir WM, and Bruce LJ. The Involvement of Cation Leaks in the Storage Lesion Red Blood Cells. Front Physiol. 2014;5;214.

W Romy, Suparyatha. WR, IGL Sidiartha dkk. Mortalitas Asidosis Metabolik Laktat dan Non Laktat di Unit Perawatan Intensif Pediatrik RSUP Sanglah. Denpasar. 2012;13(5);351-6.

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Submitted

2019-01-22

Accepted

2019-01-26

Published

2019-04-13

How to Cite

[1]
Wahyu, R., Nurulita, A. and Muhidin, R. 2019. ANALYSIS OF LACTIC AND HEMATOCRIT LEVELS OF BLOOD STORAGE IN DR. WAHIDIN SUDIROHUSODO GENERAL HOSPITAL BLOOD BANK. INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 25, 3 (Apr. 2019), 318–322. DOI:https://doi.org/10.24293/ijcpml.v25i3.1450.

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