Human Sperm Cells After Purification Using SCLB Can Be Stored at 4o, -20o, or -80oC Before Small RNA Isolation

Berliana Hamidah, Ashon Sa'adi, Rina Yudiwati


The aim of this study is to learn about the choice of storage temperature for human sperm cells after sperm purification using somatic cell lysis buffer (SCLB) before sperm small ribonucleic acid (RNA) isolation and analysis. This study was true laboratory experiment using the post-test only control group design. The samples were 13 fresh human semen that has been purified using SCLB. The sperm cells then diluted and divided into four aliquots with different treatment. First aliquot that served as a control group was immediately purified while the last three aliquots were stored for 7 days at different temperature: 4oC, -20o, and -80oC. The small RNA yields between each group then compared after the small RNA isolation and measured using microvolume spectrophotometer. The small RNA yields of control group was 49.8 (5.33-522.46) ng/106 sperm cells. It wasn’t show any significant difference with the other groups of storage temperature: 4oC, -20o, and -80oC [41.09 (7.03-1448.31); 65.95 (7.99-301.16); 76.42 (10.45-434.25); p value 0.314] with p value > α (α = 5%). This condition shows that human sperm cells after sperm purification using SCLB can be stored at 4oC, -20o, or -80oC temperatures depends on each laboratory facilities.


sperm RNA analysis; purified sperm cells; storage temperature


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