The Suitability of Compatibility Test Results with Gel Method between Diagnostic Grifols Gel Coombs and Diamed-Identification
Abstract
A compatibility test as part of a pre-transfusion test is mandatory to ensure blood compatibility between patients and
donors. Diamed-ID as the first gel-based product is commonly used as a reference for the compatibility test. The presence of
new products such as DG Gel Coombs encourages research to compare them with reference methods. This study aimed to
analyze the suitability of DG Gel Coombs to Diamed-ID in the compatibility test with the same sample. This cross-sectional
analytic observational study was conducted during November 2017-February 2018 at the Blood Transfusion Unit Dr.
Soetomo Hospital, Surabaya using blood samples (n=40), which met the inclusion and exclusion criteria. Simultaneous
testing of the two products was according to the manufacturing requirements of each product (using different LISS reagents
for red blood cell suspensions and centrifugation arrangements). The suitability of results was tested with Cohen's kappa
and significant differences with McNemar. There was a minimum suitability of DG Gel Coombs to Diamed-ID for major
compatibility tests, κ 0.307 (95% CI: -0.029-0.643), significance 0.007 (p < 0.05) and moderate for minor, κ 0,678 (95% CI:
0.454-0.903), significance <0.0001 (p < 0.05). McNemar's significance was 0.016 (p < 0.05) for major compatibility test and
0.031 (p < 0.05) for minor. Referring to Diamed-ID's results, false negatives were found on DG Gel Coombs for major
compatibility tests (n=7) and minor (n=6). The suitability of results from DG Gel Coombs and Diamed-ID is not strong for
compatibility testing.
Keywords
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DOI: http://dx.doi.org/10.24293/ijcpml.v27i1.1605
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