Analysis of MTb Rapid Molecular Test Performance Towards Microscopical Acid Fast Bacilli Examination at Labuang Baji General Hospital
DOI:
https://doi.org/10.24293/ijcpml.v28i1.1722Keywords:
Tuberculosis, AFB smear, MTB rapid molecular assayAbstract
Tuberculosis (Tb) is a global health problem, which is the third leading cause of death of all infectious diseases around the world, included Indonesia. Acid Fast Bacilli (AFB) smear and rapid molecular assay for Mycobacterium tuberculosis (MTb) are the old and new examinations required for MTb laboratory diagnosis. This study aimed to compare the performance of MTb rapid molecular assay and AFB smear in diagnosis and screening for Tb patients. This observational retrospective study used a cross-sectional approach, with a purposive sampling technique of 559 patients with suspected Tb in Labuang Baji Hospital, Makassar. This study was conducted from March 2019 to June 2019 by taking data from medical records from January 2018 to December 2018 at Labuang Baji Hospital, Makassar. Three hundred and forty-nine subjects were males (62.4%), and 210 subjects were females (37.6%). This study revealed sensitivity and specificity of 98.57% and 84.96%, respectively for MTb rapid molecular assay, and 68.65% and 99.44%, respectively for AFB smear, this shows that MTb rapid molecular assay was superior to AFB smear in diagnosing TB patients.
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Wedari NL, Pranata IW. Budayanti NN, Sukrama ID. Tuberculosis cases comparison in developed country (Australia) and developing country (Indonesia): A comprehensive review from clinical, epidemiological, and microbiological ascpects. 2021; 12(2): 421-426.
Kementerian Kesehatan RI. Badan penelitian dan pengembangan kesehatan. Laporan Nasional
RISKESDAS, Prevalensi TB. 2018; 80-90.
Jahan H, Jhora ST, Habib ZH, Yusuf MA, Ahmed I, et al. Diagnostic evaluation of GeneXpert MTB/RIF assay for the detection of rifampicin resistant Mycobacterium tuberculosis among pulmonary tuberculosis patients in Bangladesh. Journal of Tuberculosis Research, 2016; 4: 55-60.
Iram S, Zeenat A, Hussain S, Yusuf NW, Aslam M, et al. Rapid diagnosis of tuberculosis using Xpert MTB/RIF assay–report from a developing country. Journal Medicine Science, 2015; 31(1): 105-110.
Mavenyengwa RT. Evaluation of the Xpert MTB/Rif assay and microscopy for the diagnosis of
Mycobacterium tuberculosis in Namibia. Infect Dis Poverty, 2017; 6(1): 13.
Sopiyudin DM. Statistik untuk kedokteran dan kesehatan. Epidemiologi Indonesia. 6th Ed., Jakarta,
Arkans, 2018; 137-151.
Fernandes P, Ma Y, Gaeddert M, Tsacogianis T, Rodrigues P, et al. Sex and age differences in Mycobacterium tuberculosis infection in Brazil. Epidemiol Infect, 2018; 146(12): 1503-1510.
Campos LC, Rocha MVV, Willers DMC, Silva DR. Characteristics of patients with smear-negative
pulmonary Tuberculosis (TB) in a region with high TB and HIV prevalence. PLoS One, 2016; 11(1): e0147933.
Rasool G, Khan AM, Mohy-Ud-Din R M. Detection of Mycobacterium tuberculosis in AFB smear-negative sputum specimens through MTB culture and Genexpert ® MTB/RIF assay. Int J Immunopathol
Pharmacol, 2019; 33.
Sharma SK, Kohli M, Yadav RN, Chaubey J, Bhasin D, et al. Evaluating the diagnostic accuracy of Xpert
MTB/RIF assay in pulmonary tuberculosis. PLoS One, 2015; 10(10): e0141011.
Claessens J, Mathys V, Derdelincks I, Saegeman V. Case reports of a false positive results of the Xpert
MTB/RIF assay for rifampicin resistance in Mycobacterium tuberculosis. International Journal
of Clinical and Laboratory Medicine, 2017; 72(3): 195-197.
World Health Organization. Global tuberculosis report. 2017. Avaiolable from: https://doi.org/
1001/jama.2014.11450 (accessed February, 2019).
Mnyambwa NP, Ngadaya ES, Kimaro G, Kim DJ, Kazwala R, et al. Assesment of sputum smear-positive but culture negative results among newly diagnosed pulmonary tuberculosis patients in Tanzania. 2017; 10: 199-205.
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