Analysis of Platelet Counts and Leukocyte Residues at Different Storage Times in Thrombocyte Concentrate
DOI:
https://doi.org/10.24293/ijcpml.v30i2.2147Keywords:
Thrombocyte concentrate, storage times, leukocyte residues, plateletsAbstract
Thrombocyte Concentrate (TC) is one of the blood components given as therapy to patients. Ensuring the quality of TC products is essential to know if they can be transfused to patients. This study aimed to analyze platelet counts and leukocyte residues in TC products with different storage times. Cross-sectional research with a prospective cohort design was carried out at the Blood Transfusion Unit of Dr. Wahidin Sudirohusodo Hospital, Makassar, in September 2022. The study samples were ten bags of TC products produced from whole blood using the buffy coat method. The average platelet count in TC products on the 1st, 3rd, and 5th day were 50.65x109 (± 24.58); 66.24x109 (± 34.83); 47.34x109 per unit (± 37.75). Statistical tests showed no significant decrease between the 1st, 3rd, and 5th days. A meaningful decrease was obtained on the 3rd and 5th day (p < 0.05), while on the ratio of the 1st and 3rd day, there was no meaningful difference (p > 0.05). The average number of leukocytes is 0.0727x109 per unit (± 0.0659). The average number of platelets and leukocyte residues in TC products is by PERMENKES No. 91 of 2015 standards. Thrombocyte concentrate products can be used until the 5th day of storage.
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References
Harmening D. Modern blood banking and transfusion practices. 6th Ed., vol 93, Philadelphia, FA David Company. 2015; 1-40.
Yuan, Shan ZKO. Platelet transfusion: An update on indications and guidelines platelet transfusion an update on indications and guidelines platelet transfusion guidelines prophylactic therapeutic review. Clin Lab Med, 2021; 41(4): 621–634.
Bagudi Latha, Mani Chitra, Jay Ravishankar. Quality assessment of platelet concentrates prepared by platelet rich plasma plasma-platelet concentrate and buffy coat -platelet. Int J Curr Res, 2019; 11(12): 8963–6.
Permenkes. Permenkes no. 91 tahun 2015. Standar pelayanan tranfusi darah. 2015; 224–33.
WHO. The clinical use of blood. Reprod Health, 2002; 5: 221.
Greening DW, Simpson RJ, Sparrow RL. Preparation of platelet concentrates for research and transfusion purposes. Methods Mol Biol, 2017; 1619: 31–42.
Al-Hamed FS, Mahri M, Al-Waeli H, Torres J, Badran Z, Tamimi F. Regenerative effect of platelet concentrates in oral and craniofacial regeneration. Front Cardiovasc Med, 2019; 6: 1–14.
Arlt N, Rothe R, Moog R. Validation of blood counters for quality control of platelet concentrates with high platelet counts. J Lab Med, 2018; 42(5): 201–4.
Abu Kasim HS, Al-Hassan FM, Lim V. Assessment of platelet concentrate prepared from fresh and overnight held whole blood. Asian J Pharm Clin Res, 2016; 9(6): 16–20.
Setiawaty V, Marpaung E, Setiawaty V, Ritchie NK, Timana IS. Function and platelet count in platelets concentrate during the storage storage. Heal Sci J Indones, 2016; 6: 48–51.
Samad R, Abdullah AA, AP Kusriny, Arif M. Waktu penyimpanan trombosit terkait jumlah di konsentrat trombosit. Indones J Clin Pathol Med Lab, 2016; 20(3): 224.
Titania Az-Zahra. Karakteristik quality control thrombocyte concentrate metode apheresis dan platelet-rich plasma. Universitas Sriwijaya, 2020; 1-23.
Amalia Y, Samsulhadi W, Darmawati D. Perbedaan jumlah trombosit pada produk trombosit konsentrat (TC) yang berasal dari kantong darah 350 mL dan 450 mL di UTD PMI Kota Surabaya Tahun 2019. J Anal Med Biosains, 2022; 9(1): 09.
Lestariyani N, Herawati S. Perbedaan jumlah trombosit konsentrat trombosit pada penyimpanan hari I, III, V di unit donor darah PMI Provinsi Bali/RSUP Sanglah Denpasar. E-Jurnal Med Udayana, 2017; 6(3): 1–4.
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