ivana dewi, Nadjwa Zamalek Dalimoenthe, Anna Tjandrawati, Nida Suraya


Blood type phenotype incompatibility including Rhesus between donors and patients may result in cross-matching problems, triggering alloimmunization, and caused Hemolytic Transfusion Reaction (HTR). Rhesus blood type incompatibility between mother and fetus may cause Hemolytic Disease of Fetus and Newborn (HDFN). Pretransfusion phenotypic blood groups matching also reduce cost efficiently which means antibody screening is no longer needed. The purpose of the study is to find the proportion Rhesus blood type phenotypes in routine blood donors at Blood Donor Unit, Indonesian Red Cross in Bandung city, Indonesia.

The study is a descriptive and observational with cross sectional design. The study was done at Blood Donor Unit, Indonesian Red Cross in Bandung city from April 2016 to September 2017. The subjects were 142 routine blood donors, blood sampling was done simultaneously with blood donation. Rhesus antigen examination of ethylenediaminetetraacetic acid (EDTA) blood was done by gel method. Subjects characteristics were male (68%) and female (32%), with the mean age was 39 years. Examination of Rhesus antigen were found antigen D(100%), antigen e(98,6%), antigen C(97,9%), antigen c(38,7%) and antigen E(31,7%). Results of Rhesus phenotypes were DCe/DCe(61,3%), DCe/DcE(29,6%), DCe/dce(7%), DcE/DcE(1,4%) and DcE/dce(0.7%). The distribution of Rhesus blood type were affected by factors such as genetics, race, ethnicity, marriage, demography, and migration. Rhesus blood phenotypes proportion in routine blood donors at Blood Donor Unit, Indonesian Red Cross in Bandung city sorted from the most are DCe/DCe, DCe/DcE, DCe/dce, DcE/DcE and DcE/dce.

Keywords: blood donor, phenotype, Rhesus


Blood donor, phenotype, Rhesus

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Leger RM. Blood Group Terminology and Other Blood Groups. Dalam: Modern Blood Banking & Transfusion Practices. Edisi ke-6. Philadelphia: FA Davis Company; 2012. hlm. 175-91.

Quinley ED. Genetics. Dalam: Immunohematology Principles & Practice. Edisi ke-3. Baltimore: Lippincott Williams & Wilkins; 2011. hlm. 41-51.

Daniels G. Human Blood Groups. Edisi ke-3. West Sussex: Wiley-Blackwell. A John Wiley & Sons Ltd;2013. hlm. 182-236.

Kumar A, Patel MK, Chavda B, Ranjan A, Ahmad F. Hemolytic Disease of the Newborn: A study of 50 cases. IJSS. 2013;1(3).

Menuam T, Juengpichanvanich N, Charoenkwan P, Sakulwattana M, Cheepsattayakorn R, Fongsatitkul L, et al. Hemolytic disease of the fetus and newborn due to antibodies to minor red blood cell groups: two case reports. Chiang Mai Med J. 2012;51(4):119-23.

Wu KH, Chu SL, Chang JG, Shih MC, Peng CT. Haemolytic disease of the newborn due to maternal irregular antibodies in the Chinese population in Taiwan. Transfus Med. 2003;13:311-4.

Contreras M, Silva Md. The prevention and management of haemolytic disease of the newborn. J R Soc Med. 1994;87.

Asnawi AWA, Sathar J, Mohamed R, Deraman R, Kumaran S, Hamid SSA, et al. Fatal Delayed Haemolytic Transfusion Reaction and Hyperhaemolysis Syndrome in a Pregnant Woman with Sickle Cell Anaemia. Indian J Hematol Blood Transfus. 2014.

Pencatatan Darah Donor Inkompatibel. Bank Darah Rumah Sakit Hasan Sadikin. Bandung. 2016.

Sarkar BRS, Philip CJ, Mallhi SCR, Yadav DCP. Proportion of Rh phenotypes in voluntary blood donors. Med J Armed Forces India. 2013;69(4):330-4.

Flegel WA. Molecular genetics and clinical application of RH. Transfus Apher Sci. 2011;44(1):81-91.

Smart E, Armstrong B. Blood group systems. ISBT Sci Ser. 2008;3(6):75-81.

Blaney KD, Howard PR. Basic & Applied Concepts of Blood Banking and Transfusion Practices. Edisi ke-3. Missouri: Elsevier;2013. hlm. 1-18, 107-125.

Chasse M, McIntyre L, English SW, Tinmouth A, Knoll G, Wolfe D, et al. Effect of Blood Donors Characteristics on Transfusion Outcomes:A Systematic Review and Meta-Analysis. Transfus Med Rev. 2016;30:69-80.

WHO. Safe Blood and Blood Products. Modul 3: Blood Group Serology. Geneva: World Health Organization; 2009.

Downes KA, Shulman IA. Pretransfusion Testing. Roback JD, Grossman BJ, Harris T, Hillyer CD, editor. Dalam: AABB Technical Manual. Edisi ke-17. Bethesda: AABB Press; 2011. hlm. 437-59.

Setia R, Sachdeva P, Arora S, Handoo A, Kapoor M. Making Type and Screen Policy an Essential Component of Pretransfusion Testing:Need of the Hour in India. Glob J Transfus Med AATM. 2017;2:34-7.

Chaudhary R, Agarwal N. Safety of type and screen method compared to conventional antiglobulin crossmatch procedures for compatibility testing in Indian setting. Asian J Transfus Sci. 2011;5(2):157-9.

Patidar GK. Antibody Screning of Healthy Blood Donors:It's Time to Make it Mandatory. J Blood Disorders Transf. 2015;6(1).

Lamba DS, Kaur R, Basu S. Clinically Significant Minor Blood Group Antigens amongst North Indian Donor Population. Adv Hematol. 2013;2013

Lau FY, Wong R, Chan NPH, Chui CH, Ng E, Ng MHL, et al. Provision of phenotype-matched blood units: no need for pretransfusion antibody screening. Haematol. 2001;86(7):742-8.

Pachaury R, Arya DR, Mahawar NL, Bharti A, Das PK. Frequency of Rh Phenotypes in Voluntary Blood Donors. JMSCR. 2017;5(7):25083-8.

Gundrajukuppam DK, Vijaya SBK, Rajendran A, Sarella JD. Prevalence of Principal Rh Blood Group Antigens in Blood Donors at the Blood Bank of a Tertiary Care Hospital in Southern India. J Clin Diagn Res. 2016;10(5):EC07-10.

Karim F, Moiz B, Muhammad FJ, Ausat F, Khursid M. Rhesus and Kell Phenotyping of Voluntary Blood Donors:Foundation of a Donor Data Bank. J Coll Physicians Surg Pak 2015;25(10):757-60.

Chitra M, Jagannathan SY, Arumugam P, Ravishankar J. Prevalence of Rh Antigens among voluntary blood donors in Chennai, Tamil Nadu, India. Int J Res Med Sci. 2016;4(12):5360-3.

Musa RH, Ahmed SA, Hashim H, Ayob Y, Asidin NH, Choo PY, et al. Red cell phenotyping of blood from donors at the National blood center of Malaysia. ‎Asian J Transfus Sci. 2012;6(1):3-9.

Laporan Indikator Mutu. Bank Darah Rumah Sakit Hasan Sadikin. Bandung.

Laporan Kegiatan Unit Donor Darah Tahun 2016. Palang Merah Indonesia kota Bandung.

WHO. Blood Donor Selection. Guidelines on Assesing Donor Suitability for Blood Donation. Geneva, Switzerland: WHO Press;2012. hlm. 16-48.

Garg N, Singh DK, Tomar R, Singh B. Phenotype Prevalence of Blood Group Systems (ABO, Rh, Kell) in Voluntary, Healthy Donors-Experience of a Tertiary Care Hospital in Delhi, North India J Blood Disord Transfus. 2015;6(4).

Thakral B, Saluja K, Sharma RR, Marwaha N. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in north Indian blood donors. Transfus Apher Sci. 2010(43):17-22.

Dewan G. Comparative frequency and allelic distribution of ABO and Rh (D) blood groups of major tribal communities of southern Bangladesh with general population and their determinants. EJMHG. 2015;16:141-7.

Tesfaye K, Petros Y, Andargie M. Frequency distribution of ABO and Rh (D) blood group alleles in Silte Zone, Ethiopia. EJMHG. 2015;16:71-6.

Shehzad A. The Issue of Ethnicity in Pakistan: Historical Background J Pakistan Vision. 2011;12(2):124-30.

Dhawan H, Kumawat V, Marwaha N, Sharma R, Sachev S, Bansai D. Alloimmunization and autoimmunization in transfusion dependent thalassemia major patients: study on 319 patients. Asian J Transfus Sci. 2014;8:84-8.

Agnihotri A. Antibody screening with the Asian cell panel: Can we still do away with crossmatch? Asian J Transfus Sci. 2014;8:26-8.



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