NILAI DIAGNOSTIK KASET IMUNOKROMATOGRAFI SEBAGAI ALAT PENUNJANG DIAGNOSIS DEMAM BERDARAH DENGUE PADA PENDERITA DEWASA

Abstract

A rapid immunochromatographic test that incorporates blue particle conjugated with recombinant dengue envelope protein,
antihuman IgM monoclonal antibodies and antihuman IgG monoclonal antibodies (Virotec Dengue IgG/IgM XP; Indec Diagnostics,
Indonesia using technology of USA) was evaluated by using IgG and IgM captured ELISA (Dengue Duo ELISA, Panbio) which has a
99% sensitivity and a 92% specificity as the "gold standard”. This assay, cassette in shape, is performed in 15–30 minutes and detects
both immunoglobulin M (IgM) and IgG in a capture format. There were 57 paired sera of dengue haemmorhagic fever (according to
WHO 1997 criterias) and 72 sera of non dengue haemmorhagic fever patients (typhoid fever, UTI, malaria, chikungunya, hepatitis and
pneumonia). For the diagnosis of dengue virus infection, the rapid test showed a sensitivity of 98%, positive predictive value of 88%,
negative predictive value of 98% and specificity of 99%. The rapid test is a useful aid in rapid diagnosis and differentiation between
primary and secondary dengue virus infection.