PERBANDINGAN SEDIAAN BASAH DENGAN SEDIAAN GRAM HAPUSAN SEKRET VAGINA UNTUK DIAGNOSIS BACTERIAL VAGINOSIS

Abstract

Bacterial vaginosis (BV) is a clinical condition with changes in the vaginal ecosystem. Under normal conditions, the vaginal
ecosystem contains Lactobacilli microflora but in BV condition, it contains mixed microflora ie combination of anaerobic bacteria and
Gardnerella sp. There are approximately 300 cases of BV a year in the Dr. Soetomo General Hospital. We can examine vaginal fluid
microscopically to diagnose BV with wet mount and Gram stain method. Wet mount method is fast to do that clinicians can establish
the diagnosis and therapy earlier. Gram stain method is the gold standard method and more common to do in the laboratory but the
staining method can affect the result. This study aims to know the diagnostic value of wet mount method compared with Gram stain
method for BV. There were 30 subjects from the Gynaecology Outpatient Clinic in the Dr. Soetomo General Hospital. They presented
with from the leucorrhoea and fullfiled the clinical criteria for BV. We took the vaginal fluid and examined them with wet mount and
Gram stain microscopy. The percentage of agreement between wet mount and Gram stain method for BV Grade I is 66%, BV grade II
is 66%, BV grade III is 84.6%, and BV grade IV is 100%. The sensitivity of wet mount method is 85.71%, the specificity is 88.88%, the
positive predictive value and negative predictive value are 94.73% and 72.72%. Based on these data, wet mount method can replace
Gram stain method to diagnose BV microscopically in the case that there is not enough time and resource for Gram stain. The wet mount
method has good sensitivity, specificity, positive and negative predictive value. Wet mount method has a shortcoming in identifying the
microorganism, but this problem can be solved by combining this method with Gram stain method.