DIAGNOSIS MOLEKUL DAN APLIKASI DALAM PENGOBATAN HEPATITIS B & C

Abstract

In the whole world, up to now hepatitis B and C virus are still the main causes of chronic hepatitis, cirrhosis until hepatocellular
carcinoma. Most diagnosis is based on a serological examination such as the determination of antigen and antibody, for example
HbsAg, HbeAg and anti HCV. Recently, for hepatitis C examination, HCV core antigen is used for the detection of HCV infection during
the window period, chronic C hepatitis and for treatment monitoring. At present, serological assays are not sufficient to confirm the
diagnosis of hepatitis B and C due to mutations of false negative HbsAg or HbeAg results. Occult hepatitis B can occur with a negative
result of HbsAg, which cause difficulties in confirming the diagnosis and treatment as well. The success of treatment can be influenced
of both hepatitis B and hepatitis C that have genotypes. By using molecular examination, such as determination of HBV-DNA and HCVRNA,
it is expected that the problem of serological determination can be overcome. Molecular examination is not only useful for just the
diagnosis confirmation, such as for active phase and replicative determination. This sequence is also very useful as a data base prior to
the treatment of hepatitis B and hepatitis C as well as for their following success result.